ABSTRACT
OBJECTIVE@#To investigate the distribution of positive allergens in patients with allergic rhinitis in Changzhou.@*METHOD@#A total of 4242 cases with allergic symptoms and signs underwent skin prick test with 16 kinds of standardized allergens. The common allergens were found out. The samples were stratified on seven age groups to analysis the distributive characteristics of allergens responsible for each group. The samples were also stratified on children group and adults group to analysis their response degree to dust mite.@*RESULT@#2638 cases (62.19%) had the positive reaction, including male 1380 (52.3%) cases, female 1258 (47.7%) cases. Dermatophagoides farinae and dermatophagoides pteronyssinus had the highest positive ratio (85.86%, 83.55%) in inhalation group, followed cockroach (11.49%) and Humulus L. (9.82%), fungus (2.92%) in the lower positive ratio. Shrimp (5.76%) was the most common allergen in food group. The distribution of common allergens in different age groups was variable. There was significantly higher sensitivity to dust mite between children group compared to adults group (P < 0.01).@*CONCLUSION@#The study shows that Dust mite was the mostly responsible common allergen in Changzhou district. The distribution of common allergens in children group and adults group was variable.
Subject(s)
Adolescent , Adult , Aged , Animals , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Allergens , China , Epidemiology , Mites , Rhinitis, Allergic , Rhinitis, Allergic, Perennial , Diagnosis , Epidemiology , Skin TestsABSTRACT
In this study, peaT1 gene was subcloned into the Pichia pastoris expression vector pPIC9K, which contained both the methanol-inducible promoter and the transcription terminator of the AOX1 gene, resulting the plasmid pPIC9K-peaT1. The recombinant plasmid was linearized by Sal I or Bgl II and transformed into P. pastoris GS115 by electroporation method. Recombinant strain was screened by Minimal Dextrose Medium and further confirmed by PCR. The gene was in frame integrated into the Pichia genome through homologous recombination, resulting the recombinant strain. Regulated by the alpha-Factor, promoter of AOX1 gene and termination signal of yeast genomic, the recombinant protein was expressed and secreted into the supernatant. The SDS-PAGE analysis indicated that the apparent molecular weight of target protein was about 35 kD. Bioassay results showed that the inhibition rate of the expressed protein against TMV was 30.37%. The supernatant was collected and then purified by anion exchange chromatography. This protein can promote seedling growth of wheat obviously.
Subject(s)
Alternaria , Genetics , Fungal Proteins , Genetics , Pharmacology , Pichia , Genetics , Metabolism , Recombinant Proteins , Genetics , Pharmacology , TriticumABSTRACT
Objective To explore the clinical effect of mycobacterium tuberculosis determination by culture and microscopy assay from sputum specimens of pulmonary tuberculosis patients.Methods The sputum specimens were dyed with the Ziehl-Neelsen anti-acid dyeing direct microscopy and examined by BACTEC-TB 960 culture system.Results The positive rate of culture and microscopy were 36.6% and 24.6%,the difference in the positive rate between the two methods was significant(P